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Bone Marrow and Stem Stell Transplantation (Methods in by Meral Beksac

By Meral Beksac

This quantity is a compendium of state-of-the-art molecular tools for the profitable transplantation of hematopoietic stem cells. The participants are world-renown leaders within the box. They describe promising instruments for stem phone transplant study types, comparable to in vivo bioluminescence imaging. They speak about HLA typing, PCR-SSP typing, and HLA antigens. This quantity is a useful resource for biochemists, molecular biologists, and clinicians.

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13. 14. 15. 16. 17. 18. 19. 20. 33 means of pinhole SPECT in a TNBS colitis mouse model. Nucl. Med. Biol. 31, 93–101. Hildebrandt, I. J. and Gambhir, S. S. (2004) Molecular imaging applications for immunology. Clin. Immunol. 111, 210–224. , et al. (2004) Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J. Neurosci. Res. 76, 232–243. , et al. (2002) Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging.

1. Retroviral Transduction of Murine Bone Marrow and Stem Cells 1. 5-Fluorouracil (5-FU; for human injection), American Pharmaceutical Partners, Inc. NDC 63323-117-10 (50 mg/mL, 10 mL). Stock concentration 25 mg/mL in phosphate-buffered saline (PBS), aliquot, and store at –20°C. 2. Retroviral packaging cell line. 3. Retroviral expression plasmid. 4. Lipofectamine 2000 transfection reagent (Invitrogen, cat. no. 75 mL) (see Note 1). 5. Opti-MEM I reduced serum medium (Invitrogen, cat. no. 31985-070) (500 mL).

Ten Kate, F. , van Deventer, S. , and te Velde, A. A. (2005) Dedicated pinhole SPECT of intestinal neutrophil recruitment in a mouse model of dextran sulfate sodium-induced colitis. J. Nucl. Med. 46, 526–531. 5. Bennink, R. , de Jonge, W. , van Deventer, S. , and te Velde, A. A. (2004) Imaging of intestinal lymphocyte homing by Imaging Cell Fates and Function 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 33 means of pinhole SPECT in a TNBS colitis mouse model. Nucl. Med. Biol. 31, 93–101.

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