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Enzyme Biocatalysis: Principles and Applications by Andrés Illanes, Andrés Illanes

By Andrés Illanes, Andrés Illanes

This booklet used to be written with the aim of offering a valid foundation for the layout of enzymatic reactions according to kinetic ideas, but in addition to provide an up-to-date imaginative and prescient of the potentials and boundaries of biocatalysis, particularly with appreciate to fresh functions in methods of natural synthesis. the 1st 5 chapters are dependent within the type of a textbook, going from the fundamental rules of enzyme constitution and serve as to reactor layout for homogeneous platforms with soluble enzymes and heterogeneous platforms with immobilized enzymes. The final bankruptcy of the ebook is split into six sections that characterize illustrative case reviews of biocatalytic techniques of commercial relevance or capability, written via specialists within the respective fields.

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1994; Caridis and Papathanasiou 1995). Several systems considering immobilized urease have been tested (Arica 2000; Liang et al. 2000; Ayhan et al. 2002). Intracorporeal applications are far more complex: the enzyme should be directed to its target within the patient’s body and avoid the immune response. Immobilization to biocompatible supports may reduce the immune response significantly (Klein and Langer 1986). Several systems for enzyme delivery have been envisaged: microencapsulation, liposome entrapment (Chen and Wang 1998; Fonseca et al.

It is estimated that only lignocellulose derived ethanol can meet this challenge, so considerable effort is now being spent to overcome the technological limitations still prevailing (Wyman 1996; Sheehan and Himmel 1999). Among those, the requirement of more active and more stable cellulases is crucial. 015 per liter of bioalcohol. This requires significant increase in specific activity and production efficiency. Optimized combinations of the different cellulase fractions have been successfully employed (Baker et al.

2006). Forecast for the average growth rate in the next decade is about 3%/year. This figure can be higher if novel applications of specialty enzymes in the fine-chemicals and pharmaceutical industries develop (Wrotnowsky 1997; Schmid et al. 2001) Beyond industrial applications, there is an ever-increasing use of enzymes in other fields, like chemical and clinical analysis, biomedicine and research. Enzymes are potent analytical tools because of its specificity and sensibility that allows them to quantify substances at very low concentrations with minimal interference (van Brunt 1987).

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