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Gas Chromatography in Biology and Medicine by Ruth Porter

By Ruth Porter

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A. (1965~). , 205, 593. HILL,D. , and NEWELL, H. A. (19656). ,206,708-709. JAMES,A. , and MARTIN,A. J. P. (1951). Biochem. , 48, PVII (abstract). JAMES,A. , and MARTIN, A. J. P. (1952). Biochem. , 50,679-690. DISCUSSION 53 JAMES, A. , and MARTIN, A. J. P . (1956). Biochem. , 63, 138-143. URMEN, A. (1966). Adv. , 2,293-336. LANSDOWNE, R. , and LIPSKY, S. R. (1961). , 189, 571-572. LITTLEWOOD, A. B. (1960). J. scient. , 37, 185. LOVELOCK, J. E. (1958~). J. , 1, 35. LOVELOCK, J. E. (19586). , 182,1663-1664.

With a tritium source (lWmCi), operation is possible up to a temperature of lW"C, but with a nickel-63 source this can be extended to 350°C. c. operation may lead to the build-up of a contact potential at the cathode which can affect the chamber characteristics. Electron capture coefficients are dependent on the electron temperature so that the sensitivity is markedly voltage dependent. Finally, the calibration is likely to be non-linear. In D . W. c. system. Because of their increased mobility compared with the positive ions, almost all the free electrons can be collected by applying positive pulses, in excess of an amplitude of 40 V, to the anode, the pulse width being of the order 1-5 psec.

4 shows something of the importance of this factor. Figs. 4a and 4b are taken from the work of Desty, Goldup and Swanton (1959) and show a comparison of chromatograms obtained for an aromatic mixture with a 120-cm. packed column and a 14-m. capillary, both coated with 7,8-benzoquinoline. The theoretical plate height achieved with the packed column is actually superior to that of the capillary but the vastly greater value of N available in the latter case yields the separation. The properties of the solvent, however, are the really important feature since it is remarkably selective for these substances, for example, a 1 -08 for m/p-xylene.

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