By Terry Greene Sterling
Terry Greene Sterling enters the nervous ghettoes of Arizona, the gateway for almost half the nation's undocumented immigrants and the state that is the least welcoming towards them, to tell the tales of the boys, ladies, and kids who've crossed the border.
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Extra resources for Illegal: Life and Death in Arizona's Immigration War Zone
3. Agarose Gel Electrophoresis I. Erlenmeyer flask, 500 mL. 2. 2 with glacial acetic acid. 3. Ultra Pure agarose (Invitrogen) 4. Microwave oven (any supplier). 5. Electrophoresis system consisting of gel tray and comb, electrophoresis chamber, and power supply. 6. Digoxigenin-Iabeled molecular-weight DNA marker II (Roche). 7. Ethidium bromide solution (5 mg/mL in bidistilled water). 8. Ultraviolet (UV) transillumination screen. 4. 1. Restriction Endonuclease Digestion 1. 2. 3. 4. 5. 6. 7. 8. 9.
Microwave oven (any supplier). 5. Electrophoresis system consisting of gel tray and comb, electrophoresis chamber, and power supply. 6. Digoxigenin-Iabeled molecular-weight DNA marker II (Roche). 7. Ethidium bromide solution (5 mg/mL in bidistilled water). 8. Ultraviolet (UV) transillumination screen. 4. 1. Restriction Endonuclease Digestion 1. 2. 3. 4. 5. 6. 7. 8. 9. 2-mL Reaction tubes. Multiblock heater. Microcentrifuge. Disposable pipets. Restriction endonuclease Hinfl (high concentration 50 U/IlL).
4MNaOH. 8. Oven capable of l20°C temperature. 3. Preblocking, Prehybridization, and Hybridization 1. 2. 3. 4. 5. Hybridization oven with rotating bottles. Plastic wrap. 0. 0. 32 g NaCl, 100-200 /lg/mL of heat-denatured Escherichia coli DNA. 6. Hybridization solution: 10 mL of prehybridization solution complemented with 100-130 pmol of the digoxigenin-labeled oligonucleotide (GTG)s per 150 cm 2 of nylon membrane. 7. 5. 8. 1% SDS. 4. Chemiluminescent Detection of Digoxigenin-Labeled DNA 1. 5 with concentrated NaOH, autoclave at 120°C for 20 min.