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Modern Biochemical Engineering by M. N. Karim, S. L. Rivera (auth.)

By M. N. Karim, S. L. Rivera (auth.)

Contents: M.N. Karim, S.L. Rivera, castle Collins, united states: Artificial Neural Networks in Bioprocess kingdom Estimation.- G.E. Grampp, G.N. Stephanopoulos, Cambridge, united states, A. Sambanis, Atlanta, united states: Use of Regulated Secretion of Protein construction from Animal Cells: An Overview.- I. Karube, T. Takeuchi, D.J. Barnes, Tokyo: Biotechnological Reduction of CO2 Emissions.- B. Mattiasson, H. Hakanson, Lund, Sweden: Immunochemically established Assays for approach Control.- I. Endo, Wako-shi, Saitama, Japan: A Human Genome Analysis process (HUGA-I) constructed in Japan.- S. Shioya, Osaka, Japan: Optimization and keep watch over in Fed-Batch Bioreactors.- A. Fiechter, B. Sonnleitner, Zurich: Impacts of car mated Bioprocess platforms on smooth organic Research.- S. Furusaki, M. Seki, Tokyo: Use and Engineering Aspects of Immobilized Cells in Biotechnology.- J. Nielsen, Lyngby, Denmark: Modelling the expansion of Filamentous Fungi.- I. Economidis, Brussels: An assessment of the Biotechnology Research actions within the eu Community.-

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Karim and S. L. 6 ................. i........................ 4 10 0 0 I I I I~ 2 4 6 8 0 o 10 Time, h Fig. 15. Neural network test data and estimations for the T = 37 ~ batch data set where the training includes infrequent measurements of glucose concentration as an input to the net. Samples are assumed available every 2 h, and values are kept constant in between. 048. - Experimental data, -........... Estimation. 9 Biomass [] Glucose + Ethanol this procedure. 092). T h e c o n c l u s i o n w o u l d be that as m u c h i n f o r m a t i o n as is available s h o u l d be used to train the network.

Thus, maintenance of one differentiated property (regulated secretion) may be inherently linked to the maintenance of another (anchorage dependence). Ideally, one would like the cells to grow quickly and, under appropriate conditions, remain as stable, confluent monolayers for prolonged periods of time. g. insulin-producing AtT-20) have good growth properties (doubling time of 28 hours), but retaining them as stable layers of differentiated cells is difficult. g. g. hGH-producing AtT-20 cells) [17, 18].

From the viewpoint of cellular economy, such regulation would be useful in minimizing superfluous biosynthesis. For a cell culture process, in which the principal goal would be to maximize overall productivity, decoupling the regulation of secretion rate from that of biosynthesis would be certainly desirable. For a recombinant product, it is probably a straightforward task to avoid transcriptional down regulation through the use of high-level, constitutive promoters. However, the product may remain subject to regulation by secretion agonists or other factors at additional posttranscriptional steps.

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