In response to its predecessor, the thoroughly revised and elevated moment version of Modern Microbial Genetics specializes in how micro organism and bacteriophage set up and rearrange their genetic fabric via mutation, evolution, and genetic alternate to take optimum good thing about their surroundings.
The textual content is split into 3 sections: DNA Metabolism, Genetic reaction, and Genetic alternate. the 1st addresses how DNA replicates, maintenance itself, and recombines, in addition to the way it can be manipulated. the second one part is dedicated to how microorganisms have interaction with their setting, together with chapters on sporulation and tension surprise, and the ultimate part includes the most recent info on vintage trade mechanisms similar to transformation and conjugation.
- Gene Expression and Its rules
- Single-Stranded DNA Phages
- Genetic instruments for Dissecting Motility and improvement of Myxococcus xanthus
- Molecular Mechanism of Quorum Sensing
- Transduction in Gram-Negative micro organism
- Genetic techniques in micro organism without normal Genetic platforms
The editors additionally domesticate an awareness to international regulatory structures during the publication, elucidating how convinced genes and operons in micro organism, outlined as regulons, community and cooperate to fit the wishes of the bacterial telephone. With transparent appreciation for the impression of molecular genomics, this thoroughly revised and up-to-date version proves that Modern Microbial Genetics is still the benchmark textual content in its field.Content:
Chapter 1 Prokaryotic DNA Replication (pages 1–26): William Firshein
Chapter 2 DNA fix Mechanisms and Mutagenesis (pages 27–46): Ronald E. Yasbin
Chapter three Gene Expression and Its rules (pages 47–84): John D. Helmann
Chapter four Bacteriophage Genetics (pages 85–126): Burton S. Guttman and Elizabeth M. Kutter
Chapter five Bacteriophage ? and Its family (pages 127–143): Roger W. Hendrix
Chapter 6 Single?Stranded DNA Phages (pages 145–176): J. Eugene LeClerc
Chapter 7 Restriction?Modification structures (pages 177–225): Robert M. Blumenthal and Xiaodong Cheng
Chapter eight Recombination (pages 227–241): Stephen D. Levene and Kenneth E. Huffman
Chapter nine Molecular functions (pages 245–257): Thomas Geoghegan
Chapter 10 Genetics of Quorum Sensing Circuitry in Pseudomonas aeruginosa (pages 259–272): Daniel J. Hassett, Urs A. Ochsner, Teresa de Kievit, Barbara H. Iglewski, Luciano Passador, Thomas S. Livinghouse, Timothy R. McDermott, John J. Rowe and Jeffrey A. Whitsett
Chapter eleven Endospore Formation in Bacillus subtilis (pages 273–280): Charles P. Moran
Chapter 12 rigidity surprise (pages 281–287): Uldis N. Streips
Chapter thirteen Genetic instruments for Dissecting Motility and improvement of Myxococcus xanthus (pages 289–322): Patricia L. Hartzell
Chapter 14 Agrobacterium Genetics (pages 323–347): Walt Ream
Chapter 15 Two?Component legislation (pages 349–359): Kenneth W. Bayles and David F. Fujimoto
Chapter sixteen Molecular Mechanisms of Quorum Sensing (pages 361–384): Clay Fuqua and Matthew R. Parsek
Chapter 17 Bacterial Transposons—An more and more diversified crew of components (pages 385–427): Gabrielle Whittle and Abigail A. Salyers
Chapter 18 Transformation (pages 429–461): Uldis N. Streips
Chapter 19 Conjugation (pages 463–506): Ronald D. Porter
Chapter 20 The Subcellular Entities a.k.a. Plasmids (pages 507–560): Michael H. Perlin
Chapter 21 Transduction in Gram?Negative micro organism (pages 561–579): George M. Weinstock
Chapter 22 Genetic methods in micro organism without traditional Genetic platforms (pages 581–602): Carolyn A. Haller and Thomas J. DiChristina
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Extra resources for Modern Microbial Genetics, Second Edition
History of, 290±291 motility of, 305±308 msDNA from, 296±297 mutagenesis of, 303±305 sporulation of, 312±317 survival in nature of, 291 transduction in, 297±301 transposons of, 301±303 Myxobacteria, generalized transduction in, 562 Myxococcus, Escherichia coli versus, 181 Myxococcus xanthus, 291±317 carotenoids in, 307±308 constructing deletion mutants of, 303±305 cotransduction in genetic mapping of, 299, 300 fruiting bodies of, 291±294, 294±295, 312±317 genome of, 295±297 gliding by, 308±312 gliding genes in, 310±312 gliding motors in, 310 heat-resistant spores of, 316±317 motility of, 305±308, 308±312 msDNA from, 296±297 nonmotile mutants of, 310±312 overriding quorum sensing in, 269 regulatory units in, 306±307 s factors of, 305±306 sporogenesis in, 293±294 sporulation of, 312±317, 357 strains of, 295 survival in nature of, 291 transduction into, 297±301 transposons in, 295±296, 299, 301±303 vegetative growth of, 294 Myxococcus xanthus A plasmid, regulatory elements in, 306 Myxococcus xanthus strain DK101, in electroporation, 300, 300 n Myxococcus xanthus strain DK6204, constructing, 304 Myxophage Mx4 in Myxococcus xanthus cotransduction, 299 Myxococcus xanthus motility and, 310 in Myxococcus xanthus transduction, 297, 298 Myxophage Mx8 in Myxococcus xanthus cotransduction, 299 Myxococcus xanthus motility and, 310, 312 in Myxococcus xanthus transduction, 297, 298±299 Myxothiazol, from Stigmatella aurantiaca, 294 Myxovirescin (antibiotic TA), from myxobacteria, 294 N block, in sensor protein transmitter domain, 351 N gene, of bacteriophage l, 132 N protein, of bacteriophage l, 67, 132±133 N15 labeling, in proving semiconservative DNA replication, 4±5 N4mC (N4-methylcytosine) methylation, 197±198, 199 target-recognizing domains for, 209 N6mA (N6-methyladenine) methylation, 197±198, 199, 200 target-recognizing domains for, 209 NAD -dependent alcohol dehydrogenase, in Myxococcus xanthus sporulation, 315 Nae I endonuclease, operation of, 202±203 Naegleria gruberi, plasmids in, 540 Nannocystis exedens, survival in nature of, 291 NarL protein, of Escherichia coli, 372 Nathans, Daniel discovery of restriction enzymes by, 178, 179 molecular cloning and, 243 Natural competence, 450±451 NBU1 transposon, 390±392, 415±416 NBU2 transposon, 415±416 NBUs (nonreplicating Bacteroides units), as mobilizable transposons, 415±417 ndd (nuclear disruption defective) gene, in bacteriophage T4 infection, 108 Negative control, plasmids and, 510, 544 Neisseria binding in, 441 competence in, 433, 438, 439 DNA uptake in, 443 phase variation in, 77 type III restriction-modification systems in, 194 Neisseria gonorrhoeae binding in, 442 DNA uptake in, 445 phase variation in, 76 Neisseria meningitidis, restriction-modification system of, 184±185 Nematodes, reduced Pseudomonas aeruginosa virulence in, 265 Neurospora, methyltransferases in, 200 New England BioLabs, 245 N-glycosylic bonds, BER hydrolysis of, 34±35 Nicotiana glauca, Agrobacterium tumefaciens tumorigenesis in, 327 Nicotiana tabacum, inoculated with mutant Agrobacterium tumefaciens strains, 324 9 mers, in replication initiation, 6±7 Nitrogen, in proving semiconservative DNA replication, 4±5 INDEX Nitrogen fixation, 181 N-methyl-N0 -nitro-N-nitrosoguanidine (MNNG), adaptive repair of DNA damage from, 42 Noncoding lesions.
See also Gene expression repair bias toward, 33 in transformation, 431 Expressed sequence tags (ESTs), in cloning, 252 Expression vectors, table of, 584 Extracytoplasmic functions (ECF), of s factors, 63, 64, 305 F block, in sensor protein transmitter domain, 351 F conjugative plasmids, 147. See also Conjugation; Plasmid F F factor, 511. , 349 Fungal mitochondria, mutations in, 117 Fungi, plasmids of, 539±540, 543±544 Fuqua, Clay, 361 Furanones, in quorum sensing modulation, 378±379 Fusion in circular DNA recombination, 235±236 in complementation analysis, 558±559 G protein of isometric bacteriophage, 154 in phage assembly and release, 161 in single-stranded DNA phages, 147±148 G1 block, in sensor protein transmitter domain, 351 G2 block, in sensor protein transmitter domain, 351 gacA gene, in Pseudomonas aeruginosa virulence, 265 GacA response regulator, 375, 377 GacAS response regulator, 377 gal operon in bacteriophage l, 142 in conjugation, 475 in transcriptional regulation, 58 gal P1 promoter, in transcriptional regulation, 58 GAL1 transcription factor, in yeast two-hybrid systems, 255 GAL4 transcription factor, in yeast two-hybrid systems, 255 galK gene, in creating Myxococcus xanthus mutants, 304±305 GalK protein, from Myxococcus xanthus, 304±305 Galls, Agrobacterium tumefaciens and, 324±325.
See also Conjugation; Plasmid F F factor, 511. , 349 Fungal mitochondria, mutations in, 117 Fungi, plasmids of, 539±540, 543±544 Fuqua, Clay, 361 Furanones, in quorum sensing modulation, 378±379 Fusion in circular DNA recombination, 235±236 in complementation analysis, 558±559 G protein of isometric bacteriophage, 154 in phage assembly and release, 161 in single-stranded DNA phages, 147±148 G1 block, in sensor protein transmitter domain, 351 G2 block, in sensor protein transmitter domain, 351 gacA gene, in Pseudomonas aeruginosa virulence, 265 GacA response regulator, 375, 377 GacAS response regulator, 377 gal operon in bacteriophage l, 142 in conjugation, 475 in transcriptional regulation, 58 gal P1 promoter, in transcriptional regulation, 58 GAL1 transcription factor, in yeast two-hybrid systems, 255 GAL4 transcription factor, in yeast two-hybrid systems, 255 galK gene, in creating Myxococcus xanthus mutants, 304±305 GalK protein, from Myxococcus xanthus, 304±305 Galls, Agrobacterium tumefaciens and, 324±325.