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Modern Phytochemical Methods by Kurt Hostettmann, Matthias Hamburger, Maryse Hostettmann

By Kurt Hostettmann, Matthias Hamburger, Maryse Hostettmann (auth.), Nikolaus H. Fischer, Murray B. Isman, Helen A. Stafford (eds.)

This quantity comprises stories that are in response to a symposium, given th on the 30 assembly of The Phytochemical Society of North the US, held at Laval collage in Quebec urban, Canada on August 11-15, 1990. in past times twenty years, there were significant new advancements in tools which might be utilized towards the isolation, separation and constitution decision of complicated ordinary items. accordingly, the subject of this symposium, "Modem Phytochemical Methods", is a really well timed one. The organizers of the symposium well-known that it'll now not be attainable to hide intimately all new advances in phytochemical method. It was once for this reason made up our minds to stress common studies on fresh advancements of significant separation strategies comparable to excessive functionality liquid chromatography in addition to supercritical fluid chromato­ graphy. furthermore, advances in customary constitution decision tools, in most cases NMR and MS, are reviewed. different issues contain methodo­ logies of micro-sampling for isolation and research of trichome components in addition to fresh breakthroughs on biosynthetic stories of monoterpenes utilizing "enriched" basal cells of trichomes. the amount concludes with a evaluate of quantitative structure-activity courting (QSAR) stories of biologically lively common items. In bankruptcy I, okay. Hostettmann and his colleagues supply a basic evaluation of modern advancements within the separation of traditional items with significant emphasis on preparative separations of biologically energetic plant components. The authors current a comparability of droplet countercurrent chromatography (OCCC) with the hugely speedy and extra flexible centrifugal partition chromatography (CPC).

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Advances in the Isolation of Aroma Precursors........................................ Conclusion...................................................................................... 33 48 49 52 54 54 58 59 62 66 INTRODUCTION Gas chromatography (GC) is considered to be the method of choice in the analysis of volatile compounds, because of its high resolving power and the availability of universal detection using flame ionization (FID) detectors. H. , Plenum Press, New York, 1991 33 34 CHAPTER 2 However, GC is mainly an analytical method and its application is limited when preparative isolation of volatile compounds is required.

76: 51-63. , MARSTON, A. 1986. Preparative Chromatography Techniques: Applications in Natural Product Isolation. Springer Verlag, Berlin, pp. 139. HOSTETIMANN, K. 1983. Droplet countercurrent chromatography. In: Advances in Chromatography. Vol. 21. C. Giddings, E. Gruschka, 1. R. ), Marcel Dekker, New York, pp 165-185. , HOSTETTMANN, K. 1990. Centrifugal partition chromatography in the separation of natural products. Phytochemical Analysis, in press. , GREEN, B. 1979. Vacuum liquid chromatography.

CONCLUSION As the above-mentioned examples show, centrifugal partition chromatography has become an indispensable addition to the techniques available for the separation and purification of natural products. The wide range of applications, varying from apolar to very polar substances and from milligram to gram quantities, proves the versatility of this newly introduced variant of liquid-liquid chromatography. The rapidity of the method is one of the most attractive NEW DEVELOPMENTS IN THE SEPARATIONS OF NATURAL PRODUCTS 27 attributes.

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