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1987; Miller and Smith, 1989). Using a series of positive, negative or uncharged solutes, the data for their iontophoretic delivery across excised human skin were best described by a linear relationship between the logarithm of the iontophoretic permeability coefficient and the molal volume, as predicted by the ‘free volume’ model. g. insulin) than what would be predicted by the model (Yoshida and Roberts, 1993). In one study, the mobility of seven medications suitable for iontophoretic administration was investigated at pH 5, 7 and 9.
In vitro experimental techniques for iontophoresis research in the laboratory 35 However, human skin should be used where possible. The same should be true for electrically assisted percutaneous absorption studies. Human cadaver skin (HIV negative) can be obtained from a local skin bank, hospital or a national or international tissue source. The skin should preferably be frozen within a few hours of death, and can be supplied dermatomed to a specific thickness (typically 250–800 µm) or as full-thickness skin.
In these cases, the cation permselectivity of the skin may be lost, resulting in a reversal of electro-osmotic flow to the cathode-to-anode direction. , 1995; Bayon and Guy, 1996). , 1994). Using 14C-labelled mannitol, the direction of electro-osmotic flow in the anode-to-cathode direction was first confirmed and this was dramatically reduced or reversed as nafarelin was added to the anode chamber. Iontophoresis over a 12-h period resulted in delivery of nafarelin to the receptor phase, but a significant amount associated with the skin during this time and then desorbed passively over the next 12 h (Delgadocharro and Guy, 1995).