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Tobacco BY-2 Cells: From Cellular Dynamics to Omics by T. Nagata (Editor), K. Matsuoka (Editor), D. Inzé (Editor)

By T. Nagata (Editor), K. Matsuoka (Editor), D. Inzé (Editor)

The tobacco BY-2 mobilephone method is a different version mobilephone line for the research of dynamic positive aspects of plant cells. As extension of quantity fifty three, Tobacco BY-2 Cells, which offered easy features of the mobilephone procedure, this current quantity offers a wealth of recent ways. This most modern quantity within the sequence is a useful resource of knowledge for scientists in uncomplicated and utilized plant biology.

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Additional info for Tobacco BY-2 Cells: From Cellular Dynamics to Omics (Biotechnology in Agriculture and Forestry)

Sample text

Wash each well with sterile BY-2 medium to remove Agrobacteria. Repeat washing using approximately 80 ml of BY-2 medium per well. 3. Dismantle manifold. 4% Phytagel and appropriate antibiotics, in a 100 × 20 mm Falcon Petri dish). Using sterile forceps, lift filter from manifold stage and carefully tip it over pool of liquid on the Novel Approaches for Cell Cycle Analysis in BY-2 17 Phytagel plate. Close the plate and rotate with gentle shaking, spreading the cells evenly over the surface of Phytagel.

1997). Later, actin filaments accumulate in the PPB and may help to narrow the ring of microtubules (Eleftheriou and Palevitz 1992). Whether actin directly affects the position where microtubules appear or whether the effect is indirect, via for instance the control of the position of the nucleus, is not clear. Actin filaments accumulate at the PPB and align with the microtubules (Kakimoto and Shibaoka 1987; Palevitz 1987; Traas et al. 1987; Schmit and Lambert 1990). When the PPB microtubules disintegrate, the actin band disappears in most systems but remains in others (Mineyuki 1999).

Golgi-derived vesicles are targeted to the equatorial plane, where they fuse into tube-like structures. The cell plate matures first in the middle, where vesicles and tubules fuse into a network (the tubulo-vesicular network or TNV), and gradually merges into a fenestrated sheet. The refractory material that we see in a DIC image contains callose detected in the TNV and fenestrated plate. The callose is not detected elsewhere and is produced after activation of callose synthase(s) in the maturing cell plate (Samuels et al.

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